| Employees | Employer |
| | |
| 1839 | Eastern Illinois University 600 Lincoln Avenue University |
| 310 | Charleston Community School District #1 K-12 Public school district |
| 180 | Vesuvius USA North Industrial Area Ceramic fittings for steel manufacturing |
| 178 | Charleston Transitional Facility Improving the lives of individuals with developmental disabilities by providing residential and day program services |
| 135 | J.J. Collins & Sons, Inc North Industrial Area Business Forms and Commercial Web Print Manufacturer |
| 100 | Arkwright North Industrial Area Reprographical Supplies |
| 60 | Safety Storage North Industrial Area Hazaradous Storage Buildings |
| 50 | ITW Hi-Cone North Industrial Area Plastic multipak beverage carriers |
| 45 | Dow Chemical Company North Industrial Area Urethane Rigid Foam Insulation |
| 45 | Lester Building Systems 890 West State St. Manufacturing of pre-engineered, wood frame buildings |
| 38 | Consolidated Technology North Industrial Area Metal Castings |
| 37 | Eastern Illinois Precast, Inc. 20005 E. State Hwy 16 Precast concrete pipe and products |
| 30 | Wausau Homes, Inc. 750 West State Street Manufactured Homes |